Dialysis Merchandise | Thermo Fisher Scientific

April 8, 2021 0 Comments

Dialysis Products

Thermo Scientific dialysis items assist facilitate the fast and trouble-free dialysis of pattern volumes from 10 μL to 250 mL. In contrast to customary flat tubing, these modern units don’t require knots or clips that may result in leaking and pattern loss.

Pierce 96-well Microdialysis Plates and Slide-A-Lyzer Dialysis MINI Units are perfect for small volumes, Slide-A-Lyzer Dialysis Cassettes (authentic and G2) are beneficial for small to medium volumes, and Slide-A-Lyzer Dialysis Flasks are beneficial for bigger volumes. 

  • Wonderful pattern recoveries—low-binding plastic and membrane assist decrease pattern loss in comparison with filtration and resin methods
  • Handy—easy-to-grip format helps simplify pattern addition and removing with syringe and/or pipette
  • Safe—sealed membranes assist stop leakage that may happen with dialysis tubing and home made units
  • Validated—every gadget is leak-tested throughout manufacturing

Select the fitting dialysis gadget to your experiments

 

 

 

 

 

 

  Pierce 96-well Microdialysis Plates Slide-A-Lyzer MINI Dialysis Units Slide-A-Lyzer Dialysis Cassettes (authentic) Slide-A-Lyzer G2 Dialysis Cassettes Slide-A-Lyzer Dialysis Flasks
Exterior float required? No No Sure No Optionally available
Technique of pattern addition/removing Pipette Pipette Syringe Pipette or syringe Pipette
Quantity vary 10–100 μL 10 μL–2 mL 0.1–30 mL 0.25–70 mL 150–250 mL
Variety of units with totally different quantity capacities 1 3 4 5 1
Molecular weight cutoffs (MWCOs) 3.5K, 10K 2K (0.1 mL measurement solely), 3.5K, 10K, 20K 2K, 3.5K, 7K (0.1 and 0.5 mL solely), 10K, 20K 2K, 3.5K, 7K (0.1 and 0.5 mL solely), 10K, 20K 2K, 3.5K, 10K, 20K
Automation appropriate? Sure No No No No
Built-in buffer reservoir? Sure Sure, besides 0.1 mL measurement No No No
Gamma-irradiated choices? No No Sure, 10K solely Sure, 10K solely No
Molecular weight cutoff (MWCO) colour coded? No Sure Sure Sure Sure
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Be taught extra about our dialysis merchandise

2K_MWCO_graphPattern retention by the 2K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, bacitracin, tyrosine kinase peptide 1, biotin-TPKs substrate, protein kinase Ce (PKCe) peptide substrate and insulin A sequence mannequin methods (0.5-1 mg/mL) in both saline or 0.2M carbonate bicarbonate buffer, pH 9.4 have been dialyzed in a single day (17 hours) at 4°C. The quantity of retentate was estimated utilizing both the Thermo Scientific™ Pierce™ BCA Protein Assay or absorption at 360 nm (for vitamin B12).

3-5K_MWCO_graphPattern retention by the three.5K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, insulin B chain and insulin (1 mg/mL) in both saline or 0.2M carbonate bicarbonate buffer, pH 9.4 have been dialyzed in a single day (17 hours) at 4°C. The quantity of retentate was estimated utilizing both the Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

7K_MWCO_graphPattern retention by the 7K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, insulin B chain, insulin and cytochrome C(1 mg/mL) in both 0.15M sodium chloride or 0.2M carbonate bicarbonate buffer, pH 9.4 have been dialyzed in a single day (17 hours) at 4°C. The quantity of retentate was estimated utilizing both the Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

10K_MWCO_graphPattern retention by the 10K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Insulin B chain, insulin and cytochrome C (1 mg/mL) in both 0.15M sodium chloride or 0.2M carbonate bicarbonate buffer, pH 9.4 have been dialyzed in a single day (17 hours) at 4°C. The quantity of retentate was estimated utilizing the Pierce BCA Protein Assay.

20K_MWCO_graphPattern retention by the 20K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Insulin B chain, cytochrome C, lysozyme and myoglobin have been dialyzed in a single day (17 hours) at 4°C in PBS, pH 7.4. The quantity of retentate was estimated utilizing the Pierce BCA Protein Assay.


Thermo Scientific Slide-A-Lyzer Cassettes defend your treasured samples throughout dialysis and assist decrease the hassles and dangers of shedding your samples utilizing slippery tubing. They’re accessible in sizes from 10 µL to 250 mL and in a full vary of MWCOs.

 

 

 

Conventional tubing vs. Cassette
Slippery when moist
Flat tubing is troublesome to deal with when moist, growing threat of pattern loss throughout pattern addition/removing
pattern dealing with Straightforward dealing with and safe pattern supply
Plastic body and cap enable for safe pattern addition/removing
Danger of pattern loss
Pattern can leak out if clamp turns into free or falls off
pattern restoration Pattern safety
Welded membrane and leak-proof cap helps decrease the chance of pattern loss
Time consuming
Non-optimized design that sometimes requires in a single day dialysis
velocity Quick and environment friendly
Modern design with excessive floor/quantity ratio permits for extra fast dialysis than typical tubing

Comparability of Thermo Scientific Slide-A-Lyzer Cassettes with conventional dialysis tubing.

Thermo Scientific Slide-A-Lyzer Flasks Video

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