HTDialysis

March 22, 2021 0 Comments

Obtain FAQs

Ordering FAQs

What are my choices for putting an order?
What cost choices can be found?
Do you’ve got brokers in my nation?
How quickly are you able to ship my order?
Will you ship “Acquire”?
Can I specify the delivery mode?
What’s the typical supply time for worldwide orders?

Working FAQs

What’s the distinction between the HTD 96a and HTD 96b and HTD96c?
How ought to I clear the Teflon block?
Can the Teflon block be autoclaved?
Can the Teflon block be ultrasonically cleaned?
What incubation temperatures and shakers can I exploit?
How can I mark Teflon blocks uniquely and improve the legibility of the letters?
How is the Membrane Dialysis Strips Molecular Weight Reduce Off (MWCO) pore dimension decided?
How lengthy can dry membranes be saved and what’s correct membrane hydration and preparation?
How lengthy can we retailer membranes in 20% ethanol after hydration?
What volumes can be utilized within the HTD 96a/b unit?
What causes “leakage” of proteins throughout the dialysis membrane?
What causes bodily “leakage” after prolonged use?
Is there a selected expiry date for the Teflon blocks?
What’s the time required to achieve equilibrium?
What are the size and utility necessities for the HTD 96a/b?
Can the HTD 96a/b be used on robotic workstations?
What causes issue in closing the clamp?
Can I exploit radio isotopes within the HTD 96 unit?
Are you able to present a generic protein binding protocol?
What membrane pore sizes are mostly used?

Ordering FAQs

What are my choices for putting an order?

Fax a purchase order order to HTDialysis at (01) 860-464-2029
E-mail a PDF buy order
Order by means of Worldwide Scientific suppliers together with VWR Worldwide and Fisher Scientific
 
What cost choices can be found?

Financial institution examine, wire switch, or digital fund switch (EFT). 

Do you’ve got brokers in my nation? 

We shouldn’t have any unique HTDialysis brokers, nonetheless, now we have non-exclusive brokers within the following nations:       

China
Apricot Designs, Inc. China workplace
Apricot Designs (Shanghai) Co., Ltd.
Room 1008 Dehong Constructing
665 Zhangjiang Highway, Pudong New District
Shanghai 201203 China
Tel: (86) 21 5079 6338
Fax: (86) 21 5079 6228
Cellular: +86 136 8160 1596
www.apricotdesigns.com
Electronic mail       

Japan
Double Helix Worldwide, Inc.
1046 Princeton Drive, Unit 202
Marina del Rey, CA 90292
Electronic mail

or 

Wakamori Shokai Co., LTD.
6-12 Yushima 4-chome,
Bunkyo-ku, Tokyo 113-0034
Japan
Electronic mail  

or 

Funakoshi Logo
Funakoshi Co., Ltd.
9-7 Hongo 2-Chome Bunkyou-Ku, Tokyo 113-0033, Japan
Technical help
Telephone: +81-3-5684-1620
Fax: +81-3-5684-1775
Electronic mail
www.funakoshi.co.jp

Korea

Kim & Buddies Inc.,
#1703 Ace Excessive-Finish Tower eighth,
84, Gasan digital 1-ro, Geumcheon-gu,
Seoul, South Korea ZIP code : 08590
Tel: 82-2-2647-6611
Fax: 82-2-2647-6687
Electronic mail: [email protected]
www.kimnfriends.co.kr

Italy  

EOS S.r.l.
By way of Fossona 7/A, Cervarese Santa Croce
35030 – Padova – ITALIA
Telephone: +39 049 9915554  interno 244
Fax +39 049 9919469
Electronic mail

How quickly are you able to ship my order?

Orders are usually shipped inside 24-48 hours of receipt and are accompanied by an e-mail offering the buying agent the service monitoring quantity. 

Do you ship “Acquire”?

Sure, we ship “Acquire” please present an account quantity with one of many following carriers DHL, Fed Ex, or UPS. 

Can I specify the delivery mode?

Sure, we ship all USA orders by; in a single day, 2-day, or floor whereas all Worldwide orders are shipped Specific, Precedence or Worldwide Expedited. 

What’s the typical supply time for worldwide orders?

Orders are usually delivered inside 48-72 hours barring any Customs delays.  

Working FAQs

What’s the distinction between the HTD 96 a HTD 96b and HTD 96c?

HTD96a, the unique design, wanted to be saved within the open, non-compressed state. The design was modified in response to leakage detected by two clients who left the unit closed for extended intervals, ensuing within the Teflon remaining “compressed” and due to this fact sealing inefficiently.

HTD96b is our normal mannequin and has a thicker strain plate than the HTD96a. Though nonetheless advisable to be saved within the open state, it has enough strain to seal utterly and reliably even when the unit is inadvertently left closed for prolonged intervals.
The HTD96c model was designed to scale back the time required to achieve equilibrium by reducing the diffusion path size and growing the dialysis membrane floor space to pattern quantity ratio. The diminished nicely quantity design minimizes assay pattern volumes (25-75ul) to scale back reagent prices. The smaller nicely requires larger precision when pipetting.
Each the HTD96b and HTD96c Teflon blocks use the identical base clamp so if you buy one mannequin and resolve to change, you solely must buy the choice Teflon block moderately than one other full unit. If you want further details about the HTD96c, please name technical help (860-460-5283).

Well Comparison

How ought to I clear the Teflon block?

After every use the HTD dialysis block needs to be cleaned totally with a non-ionic detergent.  Disassemble the dialysis block and soak in a single day in a 2-liter beaker containing a 2% resolution of Micro 90 (VWR worldwide, Catalog # 21830-416). Rinse the Teflon bars within the 2 liter beaker adopted by holding every Teflon bar underneath a working distilled water stream.  Care should be taken to make sure that all detergent has been rinsed from the unit as residual detergent may compromise future binding research.  The Teflon block may be disinfected with a ten% v/v resolution made utilizing business Clorox bleach which is 5.25% sodium hypochlorite.  The ultimate resolution would this be 0.525% sodium hypochlorite resolution.  By no means use any abrasive or brush for cleansing the Teflon blocks as they are going to trigger micro-striations and stop efficient sealing.

Can the Teflon block be autoclaved? 

Though the present knowledge is restricted it signifies that 2 autoclave cycles trigger just some discoloration with no antagonistic results on efficiency.

Can the Teflon block be ultrasonically cleaned? 

One scientist has efficiently used the Steris Amsco® Sonic Vitality Console, Mannequin #SC1224GD with a ~1% detergent resolution (TergajetTM Low-Foaming Phosphate-Free Powdered Detergent) added manually to the cleansing chamber with a 7 minute cycle preprogrammed and the plumbed detergent setting OFF to semi-automate the washing of the Teflon blocks.  After the wash cycle the wash water is drained from the wash chamber and the chamber refilled with recent/clear scorching water.  The Teflon bars within the wash basket are manually immersed and eliminated a number of instances for the preliminary rinsing.  This guide rinsing within the wash chamber is essential as any residual detergent can adversely impacts binding outcomes.  The ultimate rinse makes use of a 30 min preprogrammed cycle utilizing the rinse/dry cycle within the rinse chamber.  This course of has been efficiently used for greater than 30 cycles with none deleterious impacts on the Teflon blocks.

What incubation temperatures and shakers can I exploit?

The dialysis block could be incubated at any desired temperature between 20ºC and 45ºC.  Equilibrium is reached extra quickly if the dialysis block is shaken through the incubation interval.  Shaking at 80-100rpm is enough utilizing any normal incubator containing an orbital or reciprocating platform shaker e.g.  Fisher Scientific (Catalog # 14-278-104) and VWR worldwide (Catalog # 47742-750 or #33998-360).  

How can I mark Teflon blocks uniquely and improve the legibility of the letters?

Use a coloured marker pen and coloration the blocks over and across the letters. The colour usually doesn’t penetrate the letters however does coloration the floor. After permitting time to dry, totally wipe the floor thereby eradicating among the coloration. This often leads to a faint coloration that contrasts with the white of the letters making them extra legible. This coloration will stay by means of a number of washes and may facilitate studying the letters.  One other benefit is that should you use completely different colours for every block you’ve got additionally shortly recognized the proper bars for every set in the event that they get blended throughout washing. 

How is the Membrane Dialysis Strips Molecular Weight Reduce Off (MWCO) pore dimension decided?

Dialysis membranes include a matrix of cross-linked polymers. The pore score, Molecular Weight Reduce Off (MWCO), is an oblique measure of the retention efficiency utilizing a sequence of normal molecules with various molecular weights after 17 hours of dialysis. The membrane MWCO is decided because the solute dimension that’s retained by at the least 90%. Nevertheless, since a solute’s permeability can be dependent upon molecular form, diploma of hydration, ionic cost and polarity, we advocate deciding on a MWCO that’s at the least half the dimensions of the MW of the species to be retained and/or twice the dimensions of the MW of the species meant to go by means of.

How lengthy can dry membranes be saved and what’s correct membrane hydration and preparation?

The dry membranes could be saved for as much as 2 years in sealed or Ziploc luggage at 4ºC or ambient temperature. Storage in such luggage prevents membranes from drying out and shedding their integrity.

Membrane hydration and preparation: 

Use solely sterile buffers to organize your membranes earlier than use. This can make sure that microbial contamination is not going to compromise membrane integrity.
By no means retailer hydrated membranes in any buffer with out an efficient anti-microbial agent e.g. 0.1% sodium azide, 1percentsodium benzoate or 1percentformaldehyde.
By no means let hydrated membranes dry as that irreversibly adjustments the pore construction and leads to lack of membrane integrity.

How lengthy can we retailer membranes in 20% ethanol after hydration?

We advocate storing them not than 4 weeks in 20% ethanol at 4ºC if the preliminary buffer was sterile. The ethanol is initially added as a result of it helps to take away any glycerin which is added to the membrane throughout manufacturing to assist promote hydration. The important thing level is that one should keep away from any bacterial progress as they could produce cellulases that modify/destroy the membranes. 

What volumes can be utilized within the HTD 96a/b?

Volumes from 25μl to 150μl can be utilized in all sides of the dialysis nicely with a most whole quantity of as much as 300ul.  Detection sensitivity will typically dictate the suitable quantity required. 

What causes “leakage” of proteins throughout the dialysis membrane?

Lack of membrane integrity throughout an experiment will manifest because the presence of proteins within the dialysate and a violation of protein mass steadiness for the nicely. This can be brought on by microbial contamination and enzymatic degradation of the cellulose membrane.  The treatment is to make sure appropriate membrane preparation and use – see above (Membrane hydration and preparation) and this contains thorough cleansing of the Teflon blocks between makes use of – (How ought to I clear the Teflon block). If “leakage” persists sterilize the Teflon block by autoclaving and repeat the experiment to substantiate that the Teflon block was contaminated and precipitated the leakage.

What causes bodily “leakage” after prolonged use?

The sealing of the unit is dependent upon the compression of the Teflon blocks by the strain plate. The most typical reason for leakage after intensive use is micro-striations on the Teflon bars resulting from inappropriate washing with abrasives or brushes. Substitute of the Teflon block cures this trigger. One other potential trigger is rusting of the disc springs within the chrome steel strain plate. Though all elements are chrome steel the grades are completely different and publicity of the strain plate to aqueous, acidic circumstances might trigger elements to rust over time. The treatment is to switch the disc springs, Cat # 1008-01. If the Teflon block in HTD 96a items is left underneath clamp strain when not in use it doesn’t “calm down” again to its unique dimension thereby leading to “leakage” when subsequent used.  This constraint doesn’t apply to HTD96b items.

Is there a selected expiry date for the Teflon blocks?

There isn’t a particular expiration interval for the Teflon blocks as will probably be depending on total use and dealing with.

What’s the time required to achieve equilibrium?

That is depending on a number of components, incubation temperature, compound construction, and shaking. Most compounds attain equilibrium in lower than 6 hours at 37ºC shaking at 80 rpm. We advocate a easy kinetic experiment with compound spiked into buffer and dialyzed in opposition to buffer to judge the equilibrium time required previous to initiating any binding experiments.  

What are the size and utility necessities for the HTD96b?

The gadget is a small bench prime, operated by hand unit with the next dimensions, 6.7” x 4.6”x 1.5” and weight is 1.2lbs. It doesn’t require any utilities or peripherals and it doesn’t require upkeep or service. The 96-well Teflon block conforms to the SBS requirements for 96 nicely plate nicely facilities. 

Can the HTD 96 be used on robotic workstations?

The HTD96b has a normal SBS 96-well base that’s appropriate with most robotic workstations. Many customers have efficiently automated their assays utilizing a wide range of business workstations, together with these from the next producers ApricotDesigns, Tecan, Hamilton, Packard, and Beckman. 

What causes issue in closing the clamp?

Inadvertently utilizing double membranes within the Teflon block as a substitute of making certain they’re separated after hydration. 

Can I exploit radio isotopes within the HTD 96 unit?

Sure radio isotopes are used and normal decontamination procedures utilizing “COUNT-OFF” from Perkin Elmer adopted by normal cleansing protocols are advisable. Nevertheless, care should be taken with the chrome steel strain plate to keep away from rusting the disc springs. If possible don’t wash or soak the strain plate. Take into account the choice of lining the gadget cavity with a skinny plastic (Saran wrap or equal meals sealing plastic wrap).  This can defend all the gadget and all you want decontaminate is the Teflon block. 

Are you able to present a generic protein binding protocol?

After assembling dialysis equipment following the Working Directions:

  1. Add 150ul of phosphate buffer or protein free serum to the receiving facet of the dialysis nicely.
    2. Add 150ul of serum (pH adjusted to 7.4) spiked with 10uM take a look at compound to the Pattern facet of the dialysis nicely.
    3. Dialyze for six hours
    4. ACN precipitate and dilute samples previous to evaluation in 1.2ml polypropylene tubes. (see directions beneath)
  2. A)    Take away 10uL from the pattern facet of the dialysis nicely and add to 1.2ml tube containing 90uL of phosphate buffer + 300ul of ACN. 

B)    Take away 90uL from the buffer facet of the dialysis nicely and add to 1.2ml tube containing 10uL of fresh serum + 300uL of ACN.

The samples are then quantitated through the Mass Spec / HPLC.  When samples are diluted/extracted on this method, all samples are in a standard matrix and the height top/space could be immediately in contrast.
All values from the Mass Spec / HPLC are corrected for pattern dilution (dilution by ACN is ignored as a result of it’s the similar for all samples).  To appropriate for dilutions, values from the pattern facet are multiplied by 10 and values from the buffer facet are multiplied by 1.1.

A typical curve could be generated to display that there’s a correlation between peak top/space and compound focus if desired.

Calculated values:
Fraction unbound = fu = focus on the buffer facet / Focus on the pattern facet.  These could be peak top or space values corrected by the dilution issue as outlined above.

What membrane pore sizes are mostly used for serum protein binding research?

12-14K MWCO              ~ 81%
6-8K MWCO                  ~13%
10K WWCO                  ~3.1%
3.5K MWCO                  ~2.4%
Different membranes have been used for particular purposes:

25K MWCO                   ~0.4%
50K MWCO                   ~0.13%

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