Spectra/Por Normal Grade Regenerated Cellulose Dialysis Membrane (Spectra/Por 1-5, 6 & 7)

Spectra/Por Standard Grade Regenerated Cellulose Dialysis Membrane (Spectra/Por 1-5, 6 & 7)
October 20, 2020 0 Comments

1 Normal Grade Regenerated Cellulose Dialysis Membrane ( 1-5, 6 & 7) advancing the science of separation

2 Desk of Contents Introduction Membrane Composition Membrane Specs Comparability of Normal RC Membranes Steel Content material in 1-6 Membranes Membrane Choice Membrane Permeability Characterization MWCO Choice Chemical Compatibility Membrane Tubing Flat Width Choice Closure Choice Membrane Preparation Membrane Dealing with and Use Pattern Restoration Membrane Storage and Shelf Life Membrane Sterilization Product Specs and Ordering Info 1-5 Normal RC Dry Dialysis Tubing Normal RC Dry Dialysis Trial Kits Normal RC Pre-wetted Dialysis Tubing & Trial Kits Normal RC Pre-treated Dialysis Tubing & Trial Kits RC Discs and Flat Sheets Prepared-to-Use Dialysis Sacks Heavy Metals Cleansing Resolution Introduction Normal Grade Regenerated Cellulose (RC) membrane is a flexible membrane out there for laboratory dialysis. It carries no mounted cost and doesn’t adsorb most solutes. Not like native cellulose, which is very crystalline and inflexible, regenerated cellulose is basically amorphous and swollen by water. Nevertheless, it nonetheless accommodates small areas of crystallinity that lock (cross-link) the structural chains in place to take care of membrane integrity. Relying on the manufacturing circumstances, these locking areas could also be quite a few. The areas between cross-links, swollen with water, act as pores allowing small enough solute molecules to go by means of the membrane. Because of the amorphous, gel-like nature of regenerated cellulose, mechanical pressure will even alter the porosity of the membrane. Membrane Composition and Specs 1, 2, 3, 4, 5, 6 and seven dialysis membranes are manufactured from pure cellulose reconstituted from cotton linters. These RC membranes carry no mounted cost and don’t adsorb most solutes. Normal RC dialysis membranes are used for basic laboratory dialysis features, equivalent to desalting, buffer change, or molecular separation. These versatile, clear membranes characteristic good chemi- ii 1

3 cal and ph resistance and excessive temperature tolerance. 1 by means of 6 include hint ranges of heavy metals and sulfides. 7 membranes have been chemically handled to reduce the heavy metallic and sulfur content material, eliminating the necessity for particular cleansing therapies. In the course of the manufacturing course of, 5 membrane is bolstered with a layer of porous paper to extend its moist energy to be used in excessive shear or torque environments, leading to a decrease permeation charge in comparison with the opposite membranes. Membrane Specs Normal Regenerated Cellulose (RC) Membrane Membrane Sort: Symmetric Regenerated Cellulose Tubing Bodily Properties: Clear, versatile ( 5 bolstered with porous paper layer) PH limits: 2-12 Advised temperature: Natural Solvent Tolerance: 60 C Good Comparability of Normal RC Membranes kd kd 3.5 kd kd kd Normal Permeability Tubing, Trial Kits, Discs & Sheets Dry with glycerin Steel Content material in 1-6 Dialysis Membranes The next are approximate focus of heavy metallic and sulfur discovered within the 1 by means of 6 membranes. Aspect Cd Cr Cu Fe Increased Permeability or giant FW Tubing, Trial Kits, Discs & Sheets Dry with glycerin Normal Permeability Tubing, Trial Kits, Discs & Sheets Dry with or with out glycerin Focus <1 ppm <1 ppm <1 ppm ppm Normal Permeability Tubing, Trial Kits, Discs & Sheets Dry with glycerin Mg Re-inforced for weight Tubing & Trial Kits Dry with out glycerin Aspect Ni Pb Zn 1, 2, 3.5, 8, 10, 15, 25 & 50 kd Pre-wetted for comfort Tubing & Trial Kits Moist in 0.05% sodium azide ppm 1, 2, 3.5, 8, 10, 15, 25 & 50 kd Pre-treated for heavy metals & sulfides Tubing & Trial Kits Moist in 0.05% sodium azide Focus 1-2 ppm 2-6 ppm 5-10 ppm 2 3

4 Membrane Choice Membrane Permeability Characterization Dialysis membranes are universally characterised by the molecular weight lower off (MWCO). Spectrum determines the MWCO of a membrane by conducting a complete dialysis check with a set of solutes of identified molecular weight. The MWCO of the membrane is set because the molecular weight of the smallest solute which is a minimum of 90% retained throughout this check (the smallest solute for which the permeation is 10% or much less). Dialysis membrane can also be characterised by the speed at which a permeable species passes by means of the membrane. A charge check could also be carried out by inserting an answer of a permeable species on one aspect of a membrane and pure solvent on the opposite. If each the answer and the solvent are properly stirred and the pure solvent is consistently modified (in order that it by no means accommodates an considerable focus of solute), a first-order charge can be noticed. A vital variable within the charge of dialysis is the molecular weight of the solute. Because the molecular weight of a permeable solute will increase, the speed of dialysis decreases. At molecular weights removed from the MWCO, the speed lower is brought on by the lower of the diffusion charge with the rising molecular weight. Because the solute s molecular weight nears the MWCO, the speed will sluggish dramatically with additional will increase in molecular weight till the molecules develop into too giant to go by means of the membrane. MWCO Choice The efficient dimension of many solute molecules can be affected by the ph and ionic energy of the answer wherein they’re dissolved. Due to this fact, the listed MWCO values must be used merely as typical and never absolute values. To determine the optimum MWCO for any utility, it could be obligatory to check a number of membranes. To maximise the speed of dialysis, the membrane with the biggest MWCO which is not going to trigger extra lack of the specified species must be used. The choosing of MWCO is predicated on the molecular weight of the macro molecules which can be going to be retained contained in the membrane and the molecular weight of the micromolecule contaminants to be eliminated. For fairly environment friendly separation by the use of dialysis with membranes, the ratio of the molecular weights of the 2 compounds to be separated must be a minimum of 25. A rule of thumb is to decide on a MWCO by choosing an MWCO worth about half of the molecular weight of the macromolecules to be retained in an effort to obtain a minimal 90% retention. Chemical Compatibility RC membranes have good chemical resistance. Variables in temperature, concentrations, durations of publicity and different components might have an effect on the usage of the membrane. It’s at all times advisable to check the membrane below your utility circumstances. These membranes are immune to the next teams: halogenated hydrocar- 4 5

5 bons, alcohols, ketones, esters, oxides and solvents containing nitrogen. They don’t seem to be advisable to be used with >25% hydrochloric, nitric and perchloric acids; 96% sulfuric acid; 1N potassium hydroxide and 10% phenol. Discuss with Spectrum s chemical compatibility desk on our web site for extra info. Membrane Tubing Flat Width Choice The choice of the tubing flat width relies on the dimensions of the pattern quantity and the dialysis reservoir. The smaller tubing (which has the next floor space to quantity ratio) will dialyze extra shortly, and bigger tubing will dialyze extra slowly as a result of longer diffusion distances concerned. For simple dealing with of the membrane tubing, the urged whole size together with closures and head house must be roughly 10 to fifteen cm. The Quantity/ ratio (ml/cm) is offered on our web site. Closure Choice It is strongly recommended to pick a closure width of 4 to 10 mm longer than the flat width of the membrane tubing. This can enable the closure to seal the tubing securely when the pattern answer is being stuffed inside. Closures can be found in two kinds of materials: polypropylene () or nylon (Common). Closures (polypropylene) The next Closures are just for use with RC membranes. 6 Normal Closure: Aids in pattern buoyancy when utilized to the highest of the membrane tubing. Weighted Closure: Comprises an embedded chrome steel weight to maintain membrane in vertical floating place when utilized to backside of membrane tubing. Magnetic Weighted Closure: Comprises an embedded magnetic stir bar, eliminating the necessity for a magnetic stir bar when the magnetic weighted closure is utilized on the backside of the membrane tubing. Common Closures (nylon) Nylon closures could also be used for every type of membrane tubing. These Common closures sink on their very own and permit the membrane tubing to drift vertically with a head house. Common Closures are usually not autoclavable. Membrane Preparation 1-6 For many functions, the membrane could be soaked in distilled water at room temperature for half-hour to take away the preservative (glycerine or sodium azide). Then, rinse the membrane completely in distilled water. If the presence of heavy metals is anticipated to trigger interferences, the membranes could be handled with Spectrum Heavy Metals Cleansing Resolution. This cleansing course of takes lower than 10 minutes to finish. The Heavy Metals Cleansing answer is a chelating rinse which strips the heavy metals from the membrane. 7

6 7 7 membrane has been chemically handled to reduce the content material of heavy metals and sulfide contaminants; subsequently, it is just essential to soak the membrane in a big quantity of deionized or distilled water for half-hour to take away the sodium azide preservant agent. Then, rinse completely with operating deionized or distilled water. Membrane Dealing with and Use The next is a basic protocol for typical dialysis. There are lots of variables that must be thought-about earlier than beginning dialysis; together with solute concentrations, pattern and dialysate volumes, solvents, chemical compatibility, temperature, and so on. Due to this fact, some application-specific modifications to the next dialysis process could also be obligatory. 1. Fill a Dialysis Reservoir with a big quantity of applicable dialysate (buffer). The dialysate quantity must be 100X the pattern quantity. (Instance: dialyze 10 ml pattern in 1 L dialysate.) 2. Lower dialysis tubing into an applicable size. Permit 10-20% additional tubing size for air head house to insure pattern buoyancy above rotating stir bar. Put together the tubing in response to the instructions to be used (see web page 7-8). 3. Open the primary Closure and clamp onto the dialysis tubing 3-5 cm from the underside. 4. Load the pattern by means of the open finish of the dialysis tubing. Clamp the second Closure a minimum of 5 cm from the highest finish of the dialysis tubing whereas permitting sufficient head house for buoyancy. 5. Place the dialysis pattern in applicable dialysis buffer. 6. Drop an appropriate-sized magnetic stir-bar into the dialysis reservoir. Place the dialysis reservoir on a stir-plate and alter the stir velocity to rotate the pattern with out pulling it down into the vortex. 7. Usually, dialysis is allowed to proceed in a single day. The dialysate (buffer) could also be modified a number of instances throughout dialysis, ensuring to permit 2-4 hours of dialysis after the final dialysate change. Observe: Samples with larger contaminant concentrations, might must dialyze for an extended length with extra frequent modifications of dialysate answer. Pattern Restoration Maintain the portion of the tubing that extends above the highest closure, open the highest closure after which pipette or pour out the pattern right into a container. 8 9

7 Membrane Storage and Shelf Life Because the helpful lifetime of dialysis membranes could be affected by storage circumstances, you will need to retailer below optimum circumstances. Dry membrane ( 1-5) must be saved in unique packaging at ambient circumstances shielded from moisture and excessive temperatures or in a polyethylene bag at 4-8 C. Correctly saved dry membrane has a shelf lifetime of 5 years. Moist membrane ( 6 and seven) and dry membrane ( 1-5) that has been wetted for storage must be saved at 4-8 C in an answer of 0.05% sodium azide, 1% sodium benzoate or 1% formaldehyde. This preservative answer must be modified a minimum of each 6 months. Correctly saved moist membrane has a shelf life of three years. steam autoclaved RC membranes must be recharacterized to compensate for any change in permeation traits or MWCO. 1-5 Normal RC Dry Dialysis Tubing Product Specs: Membrane Sort Goal MWCO 14 Flat Widths 1 Primary dialysis 6-8 kd 10, 23, 32, 40, 50, 100, Increased permeability kd 10, 25, 45, 105, Primary dialysis 3.5 kd 18, 45, 54 4 Primary dialysis kd 10, 25, 32, 45, 75 5 Strengthened for weight kd 75, 140 Membrane Sterilization The widespread methodology of membrane sterilization is publicity to ethylene oxide (EtO) gasoline. Different sterilization strategies are both irradiation or steam autoclaving. Nevertheless, Spectrum doesn’t advocate boiling or steam autoclaving Regenerated Cellulose membranes, since temperatures higher than 60 C will change the construction of membrane by reducing the permeability after sterilization. Membranes could also be autoclaved at 121 C for not more than quarter-hour (cycle must be saved as brief as potential) immersed in distilled water. Nevertheless, it’s important that Properties: Amount: Packaging: Shelf Life: Hydrophilic, symmetric porosity 15 or 30 m roll (relying on Sort and Flat Width) Dry with Glycerin (soak in water) or Dry with out Glycerin (no soaking required) 5 years from date manufactured 10 11

8 1-5 Normal RC Dry Dialysis Tubing MWCO Flat Quantity/ Glycerin Dia Width (sure/no) (m) (ml/cm) Half No. sure sure sure kd sure sure sure sure sure sure kd sure sure sure sure kd no no sure sure kd sure sure sure no kd 5 no Normal RC Dry Dialysis Trial Kits Product Specs: Membrane Sort Goal MWCO 14 Flat Widths 1 Primary dialysis 6-8 kd 10, 23, 32, 40, 50, 100, Increased permeability kd 10, 25, 45, 105, Primary dialysis 3.5 kd 18, 45, 54 4 Primary dialysis kd 32, 45, 75 5 Strengthened for weight kd 75, 140 Properties: Hydrophilic, symmetric porosity Amount: 1 m/equipment or 5 m/equipment Packaging: Dry with Glycerin (soak in water) or Dry with out Glycerin (no soaking required) Shelf Life: 5 years from date manufactured Equipment Consists of: 1m or 5m of 1, 2, 3, 4 or 5 dialysis tubing 1 Normal Closure (for buoyance) 1 weighted Closure (for orientation) 13

9 1-5 Normal RC Dry Dialysis Trial Kits MWCO Glycerin Flat Quantity/ Trial Equipment Trial Equipment Dia Width 1 meter 5 meter (sure/no) (ml/cm) size size sure T T kd kd 3.5 kd kd kd sure T T sure T T sure T T sure T T sure T T sure T T sure T T sure T T sure T T sure T T sure T T sure T T no T T no T T sure T T sure T T no T T no T T 14 6 Normal RC Pre-wetted Dialysis Tubing & Trial Kits Product Specs: 8 MWCO’s: 1 kd, 2 kd, 3.5 kd, 8 kd, 10 kd, 15 kd, 25 kd & 50 kd 12 Flat Widths: 8 mm, 12 mm, 18 mm, 24 mm, 28 mm, 32 mm, 34 mm, Properties: Amount: Packaging: Equipment Consists of: Shelf Life: 40 mm, 45 mm, 50 mm & 54 mm Hydrophilic, symmetric porosity Tubing – 10 meters/roll Trial Kits – 1 meter/roll Moist in 0.05% sodium azide 1 meter of 6 Dialysis Tubing 1 Normal Closure (for buoyancy) 1 Weighted Closure (for orientation) 5 Tubing Opening Picks 3 years from date manufactured 15

10 6 Normal RC Pre-wetted Dialysis Tubing & Trial Kits Flat Quantity/ Diameter Tubing Trial Equipment MWCO Width 10 m/size 1 m/size (ml/cm) T 1 kd 2 kd 3.5 kd 8 kd 10 kd T T T T T T T T T T T T T T 6 Normal RC Pre-wetted Dialysis Tubing & Trial Kits Flat Quantity/ Diameter Tubing Trial Equipment MWCO Width 10 m/size 1 m/size (ml/cm) 10 kd 15 kd 25 kd 50 kd T T T T T T T T T T T T T T 16 17

11 7 Normal RC Pre-treated Dialysis Tubing & Trial Kits Product Specs: 8 MWCO’s: 1 kd, 2 kd, 3.5 kd, 8 kd, 10 kd, 15 kd, 25 kd & 50 kd 12 Flat Widths: 8 mm, 12 mm, 18 mm, 24 mm, 32 mm, Properties: Amount: Packaging: Equipment Consists of: Shelf Life: 34 mm, 40 mm, 45 mm, 50 mm & 54 mm Hydrophilic, symmetric porosity Tubing – 5 meters/roll Trial Kits – 1 meter/roll Moist in 0.05% sodium azide 1 meter of seven Dialysis Tubing 1 Normal Closure (for buoyancy) 1 Weighted Closure (for orientation) 5 Tubing Opening Picks 3 years from date manufactured 7 Normal RC Pre-treated Dialysis Tubing & Trial Kits Flat Quantity/ Diameter Tubing Trial Equipment MWCO Width 5 m/size 1 m/size (ml/cm) T 1 kd 2 kd 3.5 kd 8 kd 10 kd T T T T T T T T T T T T T T 18 19

12 7 Normal RC Pre-treated Dialysis Tubing & Trial Kits (cont.) Flat Quantity/ Diameter Tubing Trial Equipment MWCO Width 5 m/size 1 m/size (ml/cm) T 10 kd T T 15 kd 25 kd 50 kd T T T T T T T T T T RC Dialysis Membrane Discs and Flat Sheets Precut membrane discs are packaged 50 per pack Flat sheet membranes are packaged 25 per pack Membrane provided dry with glycerol as a humectant 1-4 Normal RC Dialysis Membrane Discs Disc Diameter MWCO 33 mm 47 mm 100 mm kd kd kd kd Qty 50 discs 1-4 Normal RC Dialysis Membrane Flat Sheets Dimension MWCO Qty Half No kd 240 x kd 200 x sheets kd 108 x kd 150 x

13 Prepared-to-Use Dialysis Sacks Prepared-to-Use Dialysis Sacks characteristic regenerated cellulose tubing sealed at one finish with a closure and open on the different. These sacks have a funnel hooked up to the highest for simple pattern filling. Equipped in a 0.05% sodium azide preservant, sacks must be rinsed prior to make use of. Every Dialysis Sack is 60 cm in size for volumes of fifty to 400 ml. Equipped 10/package deal in three MWCO decisions 1-4 Normal RC Prepared-to-Use Dialysis Sacks MWCO Width Flat Dia Quantity/ Quantity (ml/cm) (ml) Qty kd Heavy Metals Cleansing Resolution 1, 2, 3, 4, 5, and 6 membranes include traces of heavy metals. For ultracritical work, chelate wash the membranes with Heavy Metals Cleansing answer earlier than use. 22 Half No kd kd kd Product No. Description Heavy Metals Cleansing Equipment, 8 oz. 10 sacks To position an order go to or: THE AMERICAS SpectrumLabs.com voice (800) (US & Canada) (310) (world-wide) fax (800) (US & Canada) (310) (worldwide) EUROPE SpectrumLabs.com Europe voice (0) fax (0) internet JAPAN SpectrumLabs.com Japan voice fax internet CHINA SpectrumLabs.com China voice (+86) (toll-free Mainland China) fax (+86) internet Rev

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